dc.contributor.author | Juhasz-Dora, Thomas | |
dc.contributor.author | James, Philip | |
dc.contributor.author | Evensen, Tor Hatten | |
dc.contributor.author | Lindberg, Stein-Kato | |
dc.date.accessioned | 2024-03-20T10:41:59Z | |
dc.date.available | 2024-03-20T10:41:59Z | |
dc.date.created | 2024-02-27T12:49:19Z | |
dc.date.issued | 2024 | |
dc.identifier.citation | Methods and applications in fluorescence. 2024, 12 (2), 1-7. | |
dc.identifier.uri | https://hdl.handle.net/11250/3123343 | |
dc.description.abstract | Biofluorescence in echinoderms is largely unexplored, and even though the green sea urchin Strongylocentrotus droebachiensis is a well-studied species, the presence and/or function of fluorescence remains very poorly understood. Hyperspectral imaging was conducted on adult sea urchins (N = 380) while fluorospectrometric analysis was conducted on sea urchin coelomic fluid (N = 30). Fluorescence was documented in both the spines and coelomic fluid of S. droebachiensis. Intact spines exhibited a low intensity green emission (∼550–600 nm), while broken spines averaged a high emission peak in the green spectrum (∼580 nm). Sea urchins produce a red exudate with a pronounced emission peak (∼680 nm) with a shoulder peak (∼730 nm). The sampled coelomic fluid exhibited high variability, with a majority exhibiting a low-level green fluorescence while pronounced emission peaks (N = 5) were found in the red spectrum (∼680 nm). The complex fluorescence produced by S. droebachiensis warrants further investigation on its applicability for monitoring welfare of sea urchins in aquaculture facilities. | |
dc.language.iso | eng | |
dc.title | Hidden in plain sight: hyperspectral documentation of complex biofluorescence produced by the green sea urchin (Strongylocentrotus droebachiensis) | |
dc.title.alternative | Hidden in plain sight: hyperspectral documentation of complex biofluorescence produced by the green sea urchin (Strongylocentrotus droebachiensis) | |
dc.type | Journal article | |
dc.description.version | publishedVersion | |
dc.source.pagenumber | 1-7 | |
dc.source.volume | 12 | |
dc.source.journal | Methods and applications in fluorescence | |
dc.source.issue | 2 | |
dc.identifier.doi | 10.1088/2050-6120/ad232e | |
dc.identifier.cristin | 2250213 | |
cristin.ispublished | true | |
cristin.fulltext | original | |