Improved estimation of in vitro protein digestibility of different foods using size exclusion chromatography
Rieder, Anne; Afseth, Nils Kristian; Böcker, Ulrike; Knutsen, Svein Halvor; Kirkhus, Bente; Mæhre, Hanne K; Ballance, Simon; Wubshet, Sileshi Gizachew
Peer reviewed, Journal article
Published version
Permanent lenke
https://hdl.handle.net/11250/2753544Utgivelsesdato
2021Metadata
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Originalversjon
10.1016/j.foodchem.2021.129830Sammendrag
While the harmonized INFOGEST model provides a physiologically relevant platform for simulated digestion, it needs to be combined with adequate analytical methods to enable quantification and comparison of protein digestibility in different food matrices. We have shown that size exclusion chromatography (SEC) can be used to estimate the proportion of small peptides potentially available for uptake. Combined with determination of total dissolved protein, the % of small peptides per total protein was calculated as a physiologically relevant estimate of protein digestibility (DSEC). Values for DSEC differed for casein (87.6%), chicken mince (72.6%), heated pea protein concentrate (67.8%), bread (63%), beef entrecote (57.7%) and pea protein concentrate (57.8%). In contrast to existing methods (TCA soluble protein, free NH2-groups), the proposed SEC based method gives separate insight into the two fundamental processes during protein digestion (solubilization and break-down), while maintaining the ability to rank digestibility of very different food proteins.
Tidsskrift
Food ChemistryBeslektede innførsler
Viser innførsler beslektet ved tittel, forfatter og emneord.
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INFOGEST static in vitro simulation of gastrointestinal food digestion
Brodkorb, André; Egger, Lotti; Alminger, Marie; Alvito, Paula; Assunção, Ricardo; Ballance, Simon; Bohn, Torsten; Bourlieu-Lacanal, Claire; Boutrou, Rachel; Carrière, Frédéric; Clemente, Alfonso; Corredig, Milena; Dupont, Didier; Dufour, Claire; Edwards, Catharina; Golding, Matt; Karakaya, Sibel; Kirkhus, Bente; Le Feunteun, Steven; Lesmes, Uri; Macierzanka, Adam; Mackie, Alan R.; Martins, Carla; Marze, Sébastien; McClements, David Julian; Ménard, Olivia; Minekus, Mans; Portmann, Reto; Santos, Cláudia N.; Souchon, Isabelle; Singh, R. Paul; Vegarud, Gerd Elisabeth; Wickham, Martin S. J.; Weitschies, Werner; Recio, Isidra (Journal article; Peer reviewed, 2019)Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract. These simulations have used a wide ... -
Different enzyme incorporation strategies in Atlantic salmon diet containing soybean meal: Effects on feed quality, Fish performance, nutrient digestibility and distal intestinal morphology
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Enzyme pre-treatment of soybean meal: Effects on non-starch carbohydrates, protein, phytic acid, and saponin biotransformation and digestibility in mink (Neovison vison)
Jacobsen, Hans Jakup; Kousoulaki, Katerina; Sandberg, Ann-Sofie; Carlsson, Nils-Gunnar; Ahlstrøm, Øystein; Oterhals, Åge (Journal article; Peer reviewed, 2017)