dc.contributor.author | Lima, Rita de Cássia Lemos | |
dc.contributor.author | Böcker, Ulrike | |
dc.contributor.author | McDougall, Gordon J. | |
dc.contributor.author | Allwood, J. William | |
dc.contributor.author | Afseth, Nils Kristian | |
dc.contributor.author | Wubshet, Sileshi Gizachew | |
dc.date.accessioned | 2021-09-27T07:58:31Z | |
dc.date.available | 2021-09-27T07:58:31Z | |
dc.date.created | 2021-08-06T15:20:32Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | PLOS ONE. 2021, 16 (2), 1-14. | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | https://hdl.handle.net/11250/2783611 | |
dc.description.abstract | Article Authors Metrics Comments Media Coverage Abstract 1. Introduction 2. Materials and methods 3. Results and discussion 4. Conclusion Supporting information References Reader Comments (0) Figures Abstract In this work, a new magnetic ligand fishing probe for discovery of DPP-IV inhibitory ligands was developed and it was tested as a proof of concept on the fruit extract of Vaccinium vitis-idaea (lingonberry). The ligands were shown to have appreciable dipeptidyl peptidase IV (DPP-IV) inhibitory activity (IC50: 31.8 μg mL-1).) Inhibition of DPP-IV is a well-known therapeutic approach for management of type 2 diabetes (T2D). DPP-IV was successfully immobilized onto magnetic beads and was shown to retain its catalytic activity and selectivity over a model mixture. A total of four ligands were successfully fished out and identified as cyanidin-3-galactoside (2), cyanidin-3-arabinoside (3), proanthocynidin A (4), and 10-carboxyl-pyranopeonidin 3-O-(6″-O-p-coumaroyl)-glucoside (5) using HPLC/HRMS. | |
dc.language.iso | eng | |
dc.title | Magnetic ligand fishing using immobilized DPP-IV for identification of antidiabetic ligands in lingonberry extract | |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.description.version | publishedVersion | |
dc.source.pagenumber | 1-14 | |
dc.source.volume | 16 | |
dc.source.journal | PLOS ONE | |
dc.source.issue | 2 | |
dc.identifier.doi | 10.1371/journal.pone.0247329 | |
dc.identifier.cristin | 1924448 | |
dc.relation.project | Nofima AS: 202102 | |
dc.relation.project | Norges forskningsråd: 314111 | |
dc.relation.project | Nofima AS: 11931 | |
dc.relation.project | Norges forskningsråd: 261849 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |