• norsk
    • English
  • English 
    • norsk
    • English
  • Login
View Item 
  •   Home
  • Nofima
  • Publikasjoner fra CRIStin
  • View Item
  •   Home
  • Nofima
  • Publikasjoner fra CRIStin
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Characterisation of the molecular properties of scleroglucan as an alternative rigid rod molecule to xanthan gum for oropharyngeal dysphagia

Li, Xinxin; Lu, YuDong; Adams, Gary G.; Zobel, Hanne; Ballance, Simon; Wolf, Bettina; Harding, Stephen E.
Peer reviewed, Journal article
Published version
Thumbnail
View/Open
1-s2.0-S0268005X19318375-main.pdf (1.581Mb)
URI
http://hdl.handle.net/11250/2640767
Date
2020
Metadata
Show full item record
Collections
  • Artikler / Articles [776]
  • Publikasjoner fra CRIStin [1685]
Abstract
Scleroglucan, a neutral β(1–3) glucan with β(1–6) glucan branches every third residue, is being considered as an alternative rod-like, shear thinning high molecular weight β-glucan based polysaccharide to xanthan gum for the management of patients with oropharyngeal dysphagia. It is therefore important to understand more fully its hydrodynamic properties in solution, in particular heterogeneity, molecular weight distribution and its behaviour in the presence of mucin glycoproteins. A commercially purified scleroglucan preparation produced by fermentation of the filamentous fungus Sclerotium rolfsii was analysed in deionised distilled water with 0.02% added azide. Sedimentation velocity in the analytical ultracentrifuge showed the scleroglucan preparation to be unimodal at concentrations >0.75 mg/ml which resolved into two components at lower concentration and with partial reversibility between the components. Sedimentation coefficient versus concentration plots showed significant hydrodynamic non-ideality. Self-association behaviour was confirmed by sedimentation equilibrium experiments with molecular weights between ~3 × 106 g/mol to ~5 × 106 g/mol after correcting for thermodynamic non-ideality. SEC-MALS-viscosity experiments showed a transition between a rod-shape at lower molar masses to a more flexible structure at higher masses consistent with previous observations. Sedimentation velocity experiments also showed no evidence for potentially problematic interactions with submaxillary mucin.
Journal
Food Hydrocolloids

Contact Us | Send Feedback

Privacy policy
DSpace software copyright © 2002-2019  DuraSpace

Service from  Unit
 

 

Browse

ArchiveCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsDocument TypesJournalsThis CollectionBy Issue DateAuthorsTitlesSubjectsDocument TypesJournals

My Account

Login

Statistics

View Usage Statistics

Contact Us | Send Feedback

Privacy policy
DSpace software copyright © 2002-2019  DuraSpace

Service from  Unit