dc.contributor.author | Wubshet, Sileshi Gizachhew | |
dc.contributor.author | Liu, Bingrui | |
dc.contributor.author | Kongstad, Kenneth T. | |
dc.contributor.author | Böcker, Ulrike | |
dc.contributor.author | Petersen, Malene J. | |
dc.contributor.author | Li, Tuo | |
dc.contributor.author | Wang, Junru | |
dc.contributor.author | Staerk, Dan | |
dc.date.accessioned | 2019-04-03T08:48:15Z | |
dc.date.available | 2019-04-03T08:48:15Z | |
dc.date.created | 2019-03-28T13:26:10Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Talanta: The International Journal of Pure and Applied Analytical Chemistry. 2019, 200 279-287. | nb_NO |
dc.identifier.issn | 0039-9140 | |
dc.identifier.uri | http://hdl.handle.net/11250/2593069 | |
dc.description.abstract | Plants are well-recognized sources of inhibitors for -glucosidase - a key target enzyme for management of type 2 diabetes. Recently, two advanced bioactivity-profiling techniques, i.e., ligand fishing and high-resolution inhibition profiling, have shown great promises for accelerating identification of -glucosidase inhibitors from complex plant extracts. Non-specific affinities and non-specific inhibitions are major sources of false positive hits from ligand fishing and highresolution inhibition profiling, respectively. In an attempt to minimize such false positive hits, we describe a new screening approach based on ligand fishing and high-resolution inhibition profiling for detection of high-affinity ligands and assessment of inhibitory activity, respectively. The complementary nature of ligand fishing and high-resolution inhibition profiling was explored to Manuscript for Talanta 3 identify-glucosidase inhibitory ligands from a complex mixture, and proof-of-concept was demonstrated with crude ethyl acetate extract of Ginkgo biloba. In addition to magnetic beads with a 3-carbon aliphatic linker, -glucosidase was immobilized on magnetic beads with a 21-carbon aliphatic linker; and the two different types of magnetic beads were compared for their hydrolytic activity and fishing efficiency. | |
dc.language.iso | eng | nb_NO |
dc.title | Combined magnetic ligand fishing and high-resolution inhibition profiling for identification of α-glucosidase inhibitory ligands: A new screening approach based on complimentary inhibition and affinity profiles | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | acceptedVersion | |
dc.description.version | publishedVersion | |
dc.source.pagenumber | 279-287 | nb_NO |
dc.source.volume | 200 | nb_NO |
dc.source.journal | Talanta: The International Journal of Pure and Applied Analytical Chemistry | nb_NO |
dc.identifier.doi | 10.1016/j.talanta.2019.03.047 | |
dc.identifier.cristin | 1688554 | |
dc.relation.project | Norges forskningsråd: 262308 | nb_NO |
dc.relation.project | Norges forskningsråd: 261849 | nb_NO |
cristin.unitcode | 7543,3,2,0 | |
cristin.unitname | Råvare og prosess | |
cristin.ispublished | true | |
cristin.fulltext | postprint | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |