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dc.contributor.authorRønning, Sissel Beate
dc.contributor.authorPedersen, Mona Elisabeth
dc.contributor.authorAndersen, Petter
dc.contributor.authorHollung, Kristin
dc.date.accessioned2018-02-07T07:54:38Z
dc.date.available2018-02-07T07:54:38Z
dc.date.created2013-08-14T15:28:33Z
dc.date.issued2013
dc.identifier.citationDifferentiation. 2013, 86 (1-2), 13-22.
dc.identifier.issn0301-4681
dc.identifier.urihttp://hdl.handle.net/11250/2483109
dc.description.abstractPrimary muscle cell model systems from farm animals are widely used to acquire knowledge about muscle development, muscle pathologies, overweight issues and tissue regeneration. The morphological properties of a bovine primary muscle cell model system, in addition to cell proliferation and differentiation features, were characterized using immunocytochemistry, western blotting and real- time PCR. We observed a reorganization of the Golgi complex in differentiated cells. The Golgi complex transformed to a highly fragmented network of small stacks of cisternae positioned throughout the myotubes as well as around the nucleus. Different extracellular matrix (ECM) components were used as surface coatings in order to improve cell culture conditions. Our experiments demonstrated improved proliferation and early differentiation for cells grown on surface coatings containing a mixture of both glycosaminoglycans (GAGs) and fibrous proteins. We suggest that GAGs and fibrous proteins mixed together into a composite biomaterial can mimic a natural ECM, and this could improve myogenesis for in vitro cell cultures.
dc.language.isoeng
dc.titleThe combination of glycosaminoglycans and fibrous proteins improves cell proliferation and early differentiation of bovine primary skeletal muscle cells
dc.typePeer reviewed
dc.typeJournal article
dc.description.versionsubmittedVersion
dc.source.pagenumber13-22
dc.source.volume86
dc.source.journalDifferentiation
dc.source.issue1-2
dc.identifier.doi10.1016/j.diff.2013.06.006
dc.identifier.cristin1043251
dc.relation.projectEgen institusjon: 201307
dc.relation.projectNorges forskningsråd: 224820
dc.relation.projectEgen institusjon: 4047
dc.relation.projectNorges forskningsråd: 203697
cristin.unitcode7543,3,2,0
cristin.unitnameRåvare og prosess
cristin.ispublishedtrue
cristin.fulltextpreprint
cristin.qualitycode1


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