Vis enkel innførsel

dc.contributor.authorRønning, Sissel Beate
dc.contributor.authorCarlson, Cathrine Rein
dc.contributor.authorStang, Espen
dc.contributor.authorKolset, Svein Olav
dc.contributor.authorHollung, Kristin
dc.contributor.authorPedersen, Mona Elisabeth
dc.date.accessioned2017-09-27T11:26:20Z
dc.date.available2017-09-27T11:26:20Z
dc.date.created2015-05-27T11:40:54Z
dc.date.issued2015
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11250/2457057
dc.description.abstractThe cell surface proteoglycan syndecan-4 has been reported to be crucial for muscle differentiation, but the molecular mechanisms still remain to be fully understood. During in vitro differentiation of bovine muscle cells immunocytochemical analyses showed strong labelling of syndecan-4 intracellularly, in close proximity with Golgi structures, in membranes of intracellular vesicles and finally, in the nuclear area including the nuclear envelope. Chase experiments showed that syndecan-4 was internalized from the plasma membrane during this process. Furthermore, when syndecan-4 was knocked down by siRNA more myotubes were formed, and the expression of myogenic transcription factors, β1-integrin and actin was influenced. However, when bovine muscle cells were treated with a cell-penetrating peptide containing the cytoplasmic region of syndecan-4, myoblast fusion and thus myotube formation was blocked, both in normal cells and in syndecan-4 knock down cells. Altogether this suggests that the cytoplasmic domain of syndecan-4 is important in regulation of myogenesis. The internalization of syndecan-4 from the plasma membrane during muscle differentiation and the nuclear localization of syndecan-4 in differentiated muscle cells may be part of this regulation, and is a novel aspect of syndecan biology which merits further studies.
dc.language.isoeng
dc.titleSyndecan-4 regulates muscle differentiation and is internalized from the plasma membrane during myogenesis
dc.typePeer reviewed
dc.typeJournal article
dc.description.versionpublishedVersion
dc.source.volume10
dc.source.journalPLoS ONE
dc.source.issue6
dc.identifier.doi10.1371/journal.pone.0129288
dc.identifier.cristin1244658
dc.relation.projectStiftelsen Kristian Gerhard Jebsen: SKGJ-MED-005
dc.relation.projectNorges forskningsråd: 203697
dc.relation.projectNofima AS: 4047
dc.relation.projectNorges forskningsråd: 224820
dc.relation.projectNofima AS: 201307
cristin.unitcode7543,3,2,0
cristin.unitcode7543,3,1,0
cristin.unitnameRåvare og prosess
cristin.unitnameMat og helse
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


Tilhørende fil(er)

Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel