Raman spectroscopy as a tool for characterisation of quality parameters in Norwegian grown apples during ripening
Monago-Maraña, Olga; Wold, Jens Petter; Remberg, Siv Fagertun; Sanden, Karen Wahlstrøm; Afseth, Nils Kristian
Peer reviewed, Journal article
Published version
Date
2024Metadata
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Original version
Spectrochimica Acta Part A - Molecular and Biomolecular Spectroscopy. 2024, 323 1-9. 10.1016/j.saa.2024.124903Abstract
This study shows for the first time the feasibility of Raman spectroscopy as a non-destructive method to follow the ripening process of apple fruits. Two different varieties of apples were studied: ‘Aroma’ and ‘Elstar’. By visual inspection, Raman spectra showed that the starch content was higher in ‘Elstar’ apples compared to ‘Aroma’. The degradation of starch over time could be detected in the Raman spectra, indicating that the method can be used to monitor the ripening process. The ripeness markers starch index, soluble solids content (SSC), and the sugars glucose, fructose and sucrose were determined with traditional destructive methods. Cross validated calibration models based on Raman spectroscopy were obtained for all quality parameters, and test set validation offered good results, with R2 in the range 0.4–0.86 for ‘Aroma’ and 0.4–0.95 for ‘Elstar’, respectively. The regression coefficients showed that the calibrations relied on Raman bands associated with starch and different sugars. The results suggest that Raman spectroscopy in the future could be used to determine the optimal time of harvesting and to sort apples into different degrees of ripeness.