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dc.contributor.authorAdams, Gary G.
dc.contributor.authorMeal, Andrew
dc.contributor.authorMorgan, Paul. S.
dc.contributor.authorAlsahrani, Qushmua E.
dc.contributor.authorZobel, Hanne
dc.contributor.authorLithgo, Ryan
dc.contributor.authorKok, M. Samil
dc.contributor.authorBesong, David T. M.
dc.contributor.authorJiwani, Shahwar I.
dc.contributor.authorBallance, Simon
dc.contributor.authorHarding, Stephen E.
dc.contributor.authorChayen, Naomi
dc.contributor.authorGillis, Richard B.
dc.date.accessioned2018-04-18T08:21:40Z
dc.date.available2018-04-18T08:21:40Z
dc.date.created2018-04-05T14:54:24Z
dc.date.issued2018
dc.identifier.citationPLoS ONE. 2018, 13 (3), 1-17.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11250/2494603
dc.description.abstractThe structure and function of clinical dosage insulin and its analogues were assessed. This included `native insulins' (human recombinant, bovine, porcine), `fast-acting analogues' (aspart, glulisine, lispro) and `slow-acting analogues' (glargine, detemir, degludec). Analytical ultracentrifugation, both sedimentation velocity and equilibrium experiments, were employed to yield distributions of both molar mass and sedimentation coefficient of all nine insulins. Size exclusion chromatography, coupled to multi-angle light scattering, was also used to explore the function of these analogues. On ultracentrifugation analysis, the insulins under investigation were found to be in numerous conformational states, however the majority of insulins were present in a primarily hexameric conformation. This was true for all native insulins and two fast-acting analogues. However, glargine was present as a dimer, detemir was a multi-hexameric system, degludec was a dodecamer (di-hexamer) and glulisine was present as a dimer-hexamer-dihexamer system. However, size-exclusion chromatography showed that the two hexameric fast-acting analogues (aspart and lispro) dissociated into monomers and dimers due to the lack of zinc in the mobile phase. This comprehensive study is the first time all nine insulins have been characterised in this way, the first time that insulin detemir have been studied using analytical ultracentrifugation and the first time that insulins aspart and glulisine have been studied using sedimentation equilibrium. The structure and function of these clinically administered insulins is of critical importance and this research adds novel data to an otherwise complex functional physiological protein.
dc.language.isoeng
dc.titleCharacterisation of insulin analogues therapeutically available to patients
dc.typePeer reviewed
dc.typeJournal article
dc.description.versionpublishedVersion
dc.source.pagenumber1-17
dc.source.volume13
dc.source.journalPLoS ONE
dc.source.issue3
dc.identifier.doidoi.org/10.1371/journal.pone.0195010
dc.identifier.cristin1577760
dc.relation.projectNorges forskningsråd: 262300
cristin.unitcode7543,3,1,0
cristin.unitnameMat og helse
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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