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dc.contributor.authorKumar, Rajesh
dc.contributor.authorGrønhaug, Kirsten Marie
dc.contributor.authorAfseth, Nils Kristian
dc.contributor.authorIsaksen, vidar
dc.contributor.authorDavies, Catharina de Lange
dc.contributor.authorDrogset, Jon Olav
dc.contributor.authorLilledahl, Magnus Borstad
dc.date.accessioned2018-01-31T12:21:10Z
dc.date.available2018-01-31T12:21:10Z
dc.date.created2015-10-22T10:07:40Z
dc.date.issued2015
dc.identifier.citationAnalytical and Bioanalytical Chemistry. 2015, 407 (26), 8067-8077.
dc.identifier.issn1618-2642
dc.identifier.urihttp://hdl.handle.net/11250/2480962
dc.description.abstractBiomolecular changes in the cartilage matrix during the early stage of osteoarthritis may be detected by Raman spectroscopy. The objective of this investigation was to determine vibrational spectral differences among different grades (grades I, II, and III) of osteoarthritis in human osteoarthritic cartilage, which was classified according to the International Cartilage Repair Society (ICRS) grading system. Degenerative articular cartilage samples were collected during total joint replacement surgery and were classified according to the ICRS grading system for osteoarthritis. Twelve cartilage sections (4 sections of each ICRS grades I, II, and III) were selected for Raman spectroscopic analysis. Safranin-O/Fast green was used for histological staining and assignment of the Osteoarthritis Research Society International (OARSI)grade. Multivariate principal component analysis (PCA) was used for data analysis. Spectral analysis indicates that the content of disordered coil collagen increases significantly during the early progression of osteoarthritis. However, the increase was not statistically significant during later stages of the disease. A decrease in the content of proteoglycan was observed only during advanced stages of osteoarthritis. Our investigation shows that Raman spectroscopy can classify the different stage of osteoarthritic cartilage and can provide details on biochemical changes. This proof-of-concept study encourages further investigation of fresh cartilage on a larger population using fiber-based miniaturized Raman probe for the Development of in vivo Raman arthroscopy as a potential diagnostic tool for osteoarthritis.
dc.language.isoeng
dc.titleOptical investigation of osteoarthritic human cartilage (ICRS grade) by confocal Raman spectroscopy: a pilot study
dc.typePeer reviewed
dc.typeJournal article
dc.description.versionacceptedVersion
dc.source.pagenumber8067-8077
dc.source.volume407
dc.source.journalAnalytical and Bioanalytical Chemistry
dc.source.issue26
dc.identifier.doi10.1007/s00216-015-8979-5
dc.identifier.cristin1282591
dc.relation.projectRegionale forskningsfond Midt-Norge: 46084300
dc.relation.projectNofima AS: 201309
dc.relation.projectNorges forskningsråd: 225347
cristin.unitcode7543,3,2,0
cristin.unitnameRåvare og prosess
cristin.ispublishedtrue
cristin.fulltextpostprint
cristin.qualitycode1


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