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dc.contributor.authorBagcioglu, Murat
dc.contributor.authorZimmermann, Boris
dc.contributor.authorKohler, Achim
dc.date.accessioned2017-09-29T09:03:35Z
dc.date.available2017-09-29T09:03:35Z
dc.date.created2015-09-20T23:15:44Z
dc.date.issued2015
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11250/2457447
dc.description.abstractBackground Analysis of pollen grains reveals valuable information on biology, ecology, forensics, climate change, insect migration, food sources and aeroallergens. Vibrational (infrared and Raman) spectroscopies offer chemical characterization of pollen via identifiable spectral features without any sample pretreatment. We have compared the level of chemical information that can be obtained by different multiscale vibrational spectroscopic techniques. Methodology Pollen from 15 different species of Pinales (conifers) were measured by seven infrared and Raman methodologies. In order to obtain infrared spectra, both reflectance and transmission measurements were performed on ground and intact pollen grains (bulk measurements), in addition, infrared spectra were obtained by microspectroscopy of multigrain and single pollen grain measurements. For Raman microspectroscopy measurements, spectra were obtained from the same pollen grains by focusing two different substructures of pollen grain. The spectral data from the seven methodologies were integrated into one data model by the Consensus Principal Component Analysis, in order to obtain the relations between the molecular signatures traced by different techniques. Results The vibrational spectroscopy enabled biochemical characterization of pollen and detection of phylogenetic variation. The spectral differences were clearly connected to specific chemical constituents, such as lipids, carbohydrates, carotenoids and sporopollenins. The extensive differences between pollen of Cedrus and the rest of Pinaceae family were unambiguously connected with molecular composition of sporopollenins in pollen grain wall, while pollen of Picea has apparently higher concentration of carotenoids than the rest of the family. It is shown that vibrational methodologies have great potential for systematic collection of data on ecosystems and that the obtained phylogenetic variation can be well explained by the biochemical composition of pollen. Out of the seven tested methodologies,the best taxonomical differentiation of pollen was obtained by infrared measurements on bulk samples, as well as by Raman microspectroscopy measurements of the corpus region of the pollen grain. Raman microspectroscopy measurements indicate that measurement area, as well as the depth of focus, can have crucial influence on the obtained data.
dc.language.isoeng
dc.titleA Multiscale Vibrational Spectroscopic Approach for Identification and Biochemical Characterization of Pollen
dc.typePeer reviewed
dc.typeJournal article
dc.description.versionpublishedVersion
dc.source.volume10
dc.source.journalPLoS ONE
dc.source.issue9
dc.identifier.doi10.1371/journal.pone.0137899
dc.identifier.cristin1265823
dc.relation.projectNofima AS: 201309
dc.relation.projectNorges forskningsråd: 225347
dc.relation.projectNofima AS: 201302
dc.relation.projectNorges forskningsråd: 225096
cristin.unitcode7543,3,2,0
cristin.unitnameRåvare og prosess
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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