The PBMC transcriptome profile after intake of oxidized versus high-quality fish oil: an explorative study in healthy subjects
Myhrstad, Mari; Ottestad, Inger; Günther, Clara-Cecilie; Ryeng, Einar; Holden, Marit; Nilsson, Astrid; Brønner, Kirsti Wettre; Kohler, Achim; Borge, Grethe Iren Andersen; Holven, Kirsten Bjørklund; Ulven, Stine Marie
Journal article
Date
2016Metadata
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Abstract
Background: Marine long-chain polyunsaturated fatty acids are susceptible to oxidation, generating a range ofdifferent oxidation products with suggested negative health effects. The aim of the present study was to utilizesensitive high-throughput transcriptome analyses to investigate potential unfavorable effects of oxidized fish oil (PV:18 meq/kg; AV: 9) compared to high-quality fish oil (PV: 4 meq/kg; AV: 3).Methods: In a double-blinded randomized controlled study for seven weeks, 35 healthy subjects were assigned to8 g of either oxidized fish oil or high quality fish oil. The daily dose of EPA+DHA was 1.6 g. Peripheral bloodmononuclear cells were isolated at baseline and after 7 weeks and transcriptome analyses were performed with theilluminaHT-12 v4 Expression BeadChip.Results: No gene transcripts, biological processes, pathway or network were significantly changed in the oxidizedfish oil group compared to the fish oil group. Furthermore, gene sets related to oxidative stress and cardiovasculardisease were not differently regulated between the groups. Within group analyses revealed a more prominenteffect after intake of high quality fish oil as 11 gene transcripts were significantly (FDR < 0.1) changed from baselineversus three within the oxidized fish oil group.Conclusion: The suggested concern linking lipid oxidation products to short-term unfavorable health effects maytherefore not be evident at a molecular level in this explorative study.Trial registration: ClinicalTrials.gov, NCT01034423
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